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You are here Measurements | Principles  
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Principles of diagnostic DNA-image-cytometry

quarter DNA-image-cytometry comprises measurement of DNA-contents of nuclei, fixed on glass slides and specifically stained with visible dyes on images of video- or digital- cameras by measurement of their integrated optical densities

 
workstation
Moticyte-DNA system for interactive nuclear DNA-measurements
 
quarter Specific stoechiometric staining of nuclear DNA on slides with pararosaniline (pink) or thionine (blue) acc. to Robert Feulgen (1923)
quarter Microdensitometric measurements of Integrated Optical Densities (IODs)
in >300 nuclei
quarter Internal calibration with normal nuclei with diploid chromosomal set (reference cells)
quarter Graphic representation of nuclear DNA-values in frequency histograms
quarter Application of diagnostic algorithms
 
cytological slides
Different types of cytological slides, stained according to Feulgen. Regions of Interest (ROIs), containing relevant cells according to previous microscopical inspection, are marked with felt-tip pen”
 
pararosanilinepararosaniline
1. Feulgen-stained (pararosaniline) rat hepatocytes
2. Segmented Feulgen-stained nuclei for DNA-measurement
 
 
 
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Principles DNA-Image-
Cytometry